Fundamentals of Plant Biochemistry and Biotechnology
Fundamentals of Plant Biochemistry and Biotechnology is the basic book that give complete information on of plant biochemistry, plant metabolic processes, genetic engineering, and biotechnological applications in agriculture. If you are looking for Fundamentals of Plant Biochemistry and Biotechnology notes you are at right place. Here you can find all BSc Agriculture notes at single platform. We have updated Plant Biotechnology notes as per the Latest ICAR 5th dean committee syllabus. You can find details about Fundamentals of Plant Biochemistry and Biotechnology 1st semester notes here.
| Name of PDF | Fundamentals of Plant Biochemistry and Biotechnology |
| Number of pages | 215 |
| PDF Size | 11.5 MB |
| Syllabus | As per ICAR 5th dean committee |
| Course | BSc Agriculture |
| Semester | 1st semester |
| Language | English |
| Notes Source | ICAR, TNAU |
Fundamentals of Plant Biochemistry and Biotechnology Syllabus -Credit = 3(2+1)
Fundamentals of Plant Biochemistry and Biotechnology Theory Syllabus
Importance of Biochemistry. Properties of Water, pH and Buffer. Carbohydrate: Importance and classification. Structures of Monosaccharides, Reducing and oxidizing properties of Monosaccharides, Mutarotation; Structure of Disaccharides and Polysaccharides. Importance and classification; Structures and properties of fatty acids; storage lipids and membrane lipids. Proteins: Importance of proteins and classification; Structures, titration and zwitterions nature of amino acids; Structural organization of proteins. Enzymes: General properties; Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes. Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure. Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain. Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids. Concepts and applications of plant biotechnology: Scope, organ culture, embryo culture, cell suspension culture, callus culture, anther culture, pollen culture and ovule culture and their applications; Micro-propagation methods; organogenesis and embryogenesis, Synthetic seeds and their significance; Embryo rescue and its significance; somatic hybridization and cybrids; Somaclonal variation and its use in crop improvement; cryo-preservation; Introduction to recombinant DNA methods: physical (Gene gun method), chemical (PEG mediated) and Agrobacterium-mediated gene transfer methods; Transgenics and its importance in crop improvement; PCR techniques and its applications; RFLP, RAPD, SSR; Marker Assisted Breeding in crop improvement; Biotechnology regulations.
Fundamentals of Plant Biochemistry and Biotechnology Practical Syllabus
Preparation of solution, pH & buffers, Qualitative tests of carbohydrates and amino acids. Quantitative estimation of glucose/ proteins. Titration methods for estimation of amino acids/lipids, Effect of pH, temperature and substrate concentration on enzyme action, Paper chromatography/ TLC demonstration for separation of amino acids/ Monosaccharides. Sterilization techniques. Composition of various tissue culture media and preparation of stock solutions for MS nutrient medium. Callus induction from various explants. Micro-propagation, hardening and acclimatization. Demonstration on isolation of DNA. Demonstration of gel electrophoresis techniques and DNA finger printing.
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